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    ELISA試劑盒多聚合酶g抗體免費(fèi)檢測

    發(fā)布時(shí)間: 2014-04-25  點(diǎn)擊次數(shù): 1298次

    ELISA試劑盒多聚合酶g抗體英文名稱  Anti-PAPOG 
    中文名稱  多聚合酶g抗體 
    別    名  PAPOLG 
    濃    度  1mg/1ml 
    規(guī) 格  0.2ml/200μg
    抗體來源  Rabbit  
    克隆類型  polyclonal 
    交叉反應(yīng)  Human, Mouse, Rat, Dog, Pig, Cow, Horse 
    產(chǎn)品類型  一抗    
    研究領(lǐng)域  
    ELISA試劑盒多聚合酶g抗體蛋白分子量  predicted molecular weight: 70kDa 
    性    狀  Lyophilized or Liquid 
    免 疫 原  KLH conjugated synthetic peptide derived from human PAPOG 
    亞    型  IgG 
    純化方法  affinity purified by Protein A 
    儲(chǔ) 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
    產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
    (石蠟切片需做抗原修復(fù)) 
     not yet tested in other applications.
     optimal dilutions/concentrations should be determined by the end user.  
    保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
    Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
    ELISA試劑盒多聚合酶g抗體產(chǎn)品介紹 This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008].
    Function : Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
    Subunit : Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.
    Subcellular Location : Nucleus.
    Tissue Specificity : Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors.
    Post-translational modifications : Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity. 
    Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF. 
    Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex. 
    Similarity : Belongs to the poly(A) polymerase family.
    Database links : UniProtKB/Swiss-Prot: Q9BWT3.2

     

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